FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g4_ov.351000000b873b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g4_ov.351000000b873b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154167
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC185270.5873817080706253TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC36000.026.52030244-45
TATGCCG37100.025.86242746-47
CGTATGC38150.025.27472144-45
TCGTATG38150.025.2124742-43
ATGCCGT39500.024.35116646-47
CCTTGTA40100.023.92606432-33
CCAGTCA40350.023.8366826-27
GAGCACA80300.023.658929
GCTTGAA41650.023.14988158-59
AGTCACC41900.023.1249328-29
CAGTCAC41600.023.12043626-27
ACCTTGT42100.023.01507232-33
GCCGTCT41400.023.00340348-49
TGCTTGA42550.022.9392958-59
CCGTCTT42300.022.90607350-51
AGAGCAC84350.022.5229578
CTCGTAT42950.022.45007542-43
GTCACCT43400.022.2709628-29
CTTGTAA44650.021.75419434-35
GTAATCT43500.021.72893136-37
Produced by FastQC (version 0.11.2)