FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g4_fb.351000000b8711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g4_fb.351000000b8711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195092
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC214180.6703406349488528TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC38800.028.22037544-45
ATGCCGT39850.027.41891746-47
CCGTCTT40000.027.31224850-51
TCGTATG40250.027.2037442-43
ACGGCTA41600.026.37265632-33
GAGCACA85700.025.1595849
AGAGCAC86900.025.0307678
GCCGTCT44650.024.41638848-49
CACGGCT45850.024.0834630-31
GTATGCC47100.024.05420944-45
GGCTACA46300.023.84938634-35
CTCGTAT46150.023.72590442-43
TATGCCG47350.023.62771446-47
GTCACGG46250.023.61844428-29
TCACGGC47500.023.59682730-31
CGGCTAC47850.023.07683632-33
CTTGAAA48800.022.77464760-61
CAGTCAC48350.022.54350526-27
AGTCACG49100.022.48933828-29
GCTTGAA49400.022.40258858-59
Produced by FastQC (version 0.11.2)