FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g4_br.351000000b8704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g4_br.351000000b8704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4809521
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC268760.5588082472246196TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC47950.027.33938430-31
TAGCTTA48650.027.09245534-35
ACTAGCT48600.027.02259832-33
TCACTAG48600.026.97373230-31
CTAGCTT49450.026.75020632-33
GTATGCC49950.026.38899244-45
TATGCCG52950.025.1189246-47
GTCACTA52850.024.8495428-29
AGCTTAT54950.024.0727534-35
GCTTATC57350.023.35545736-37
CGTATGC56900.023.29095344-45
CCAGTCA57700.023.00715426-27
TCGTATG58100.022.97341342-43
TGCTTGA58350.022.91473258-59
ATGCCGT58850.022.64098446-47
AGTCACT59250.022.52610828-29
GCCGTCT58500.022.49177648-49
GAGCACA119750.022.170839
CTTATCT60250.022.15246236-37
CCGTCTT61750.021.53798750-51
Produced by FastQC (version 0.11.2)