FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g3_ov.351000000b86e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g3_ov.351000000b86e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189329
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC173240.5431863567540383TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC34550.024.81596444-45
TATGCCG36650.023.97764846-47
CGTATGC36600.023.81535144-45
TCGTATG38750.022.12606242-43
TGCTTGA40150.022.0621458-59
ATGCCGT38950.021.95198846-47
CCGTCTT40100.021.8536250-51
CCAGTCA41350.021.30668626-27
GCTTGAA40600.021.29117858-59
GCCGTCT40300.021.21579248-49
GAGCACA81950.021.2129029
AGTCACG42550.020.59417228-29
AGAGCAC85900.020.3480438
CAGTCAC43250.020.09613426-27
CAGATCT43150.020.08830336-37
CTCGTAT44250.019.85898642-43
GATCAGA46000.019.41189634-35
CTGCTTG46150.019.04004756-57
CTTGAAA46700.019.01891960-61
ATCAGAT46650.018.6323434-35
Produced by FastQC (version 0.11.2)