FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g3_fb.351000000b86cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g3_fb.351000000b86cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752723
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC83430.4760021977231998TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17350.027.6535944-45
TATGCCG19050.026.18476746-47
ATGCCGT18750.026.09698546-47
GCCGTCT19900.024.70576548-49
TCGTATG19900.024.34875142-43
CCGTCTT20400.024.0974850-51
GTCACAC20400.023.86295328-29
TCACACT20650.023.80404730-31
CACACTT20550.023.4576630-31
GTATGCC21700.022.76686544-45
GAGCACA43150.022.5633479
ACACTTG21750.022.38180432-33
AGAGCAC43350.022.1305778
AGTCACA22950.021.31498528-29
TCTGCTT22950.021.0063756-57
CAGTCAC23300.020.99510226-27
CTCGTAT23300.020.89765442-43
CTGCTTG23450.020.65974656-57
CCAGTCA23500.020.61431926-27
CTCCAGT23950.020.5247424-25
Produced by FastQC (version 0.11.2)