FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g3_br.351000000b86a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g3_br.351000000b86a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4793028
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC289140.6032512224005367TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC57550.025.04896444-45
TATGCCG61300.023.32386646-47
TGCTTGA64800.022.3932558-59
CGTATGC65400.021.86075444-45
ATGCCGT66400.021.78280346-47
TCGTATG66650.021.62892542-43
CAGATCA66200.021.55912834-35
CCGTCTT66150.021.50553150-51
GCCGTCT66550.021.41195948-49
GCTTGAA67800.021.29731458-59
ACCAGAT68650.020.8243132-33
CTGCTTG71300.020.3195456-57
AGTCACC70950.020.18271428-29
AGATCAT72400.020.04090534-35
GAGCACA144600.020.0355689
CACCAGA71850.019.96295530-31
CCAGTCA71850.019.59939226-27
CAGTCAC73550.019.53382926-27
TCACCAG73800.019.46765730-31
CTCGTAT75150.019.18253942-43
Produced by FastQC (version 0.11.2)