FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g2_ov.351000000b867f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g2_ov.351000000b867f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163185
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC189920.8779646678393203TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC30750.029.35013644-45
TATGCCG31200.029.23333546-47
ATGCCGT33100.027.19649946-47
CGTATGC34000.027.10344144-45
GCTTGAA34300.026.5201758-59
TGCTTGA35000.026.19334258-59
CCGTCTT34950.026.16195350-51
GCCAATA35750.025.84014734-35
GCCGTCT35550.025.32102648-49
TCGTATG35900.025.205942-43
CTCGTAT37400.024.51248442-43
CACGCCA36850.024.48823530-31
AGTCACG38350.024.1496428-29
ACGCCAA39350.023.47611832-33
TCACGCC39350.023.47557830-31
CAGTCAC38550.023.40779926-27
CCAGTCA39950.022.88470526-27
TGCCGTC40300.022.8080248-49
CTTGAAA40900.022.76325660-61
CGCCAAT39900.022.7358932-33
Produced by FastQC (version 0.11.2)