FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g2_fb.351000000b8662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g2_fb.351000000b8662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145467
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC173250.8075164987389691TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT30700.029.32166546-47
TCGTATG31000.029.19046242-43
CGTATGC30500.029.12389444-45
GCCGTCT32050.027.86264248-49
CCGTCTT32550.027.14120750-51
CACAGTG33300.027.02787632-33
ACAGTGA33600.026.64520334-35
AGTGATC33850.026.30871636-37
ACACAGT35000.025.78294432-33
TATGCCG34850.025.6255346-47
GTATGCC36300.025.58278544-45
GTCACAC35650.025.579328-29
CTCGTAT35150.025.2710942-43
CAGTGAT36100.025.12887834-35
CACACAG36100.025.12887830-31
TCTCGTA36000.024.93598440-41
GCTTGAA37100.024.83676558-59
TCTGCTT36700.024.46036356-57
CTGCTTG37300.024.38524656-57
CTTGAAA37900.024.18746460-61
Produced by FastQC (version 0.11.2)