FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g2_br.351000000b863c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g2_br.351000000b863c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387614
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC121850.8781260494633234TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC18900.031.16528744-45
TATGCCG20250.029.2048946-47
TGCTTGA20250.028.85198258-59
ATGCCGT20900.028.75116546-47
ACTGACC20500.028.38120732-33
CGTATGC20450.028.33855844-45
TCGTATG21200.028.00821142-43
GCTTGAA21550.027.77274958-59
CTGCTTG21400.027.63347656-57
CCAATCT20950.027.43251836-37
TGACCAA21400.026.96566634-35
CTGACCA21900.026.45844732-33
CACTGAC22100.026.3264630-31
GCCGTCT22200.026.31721348-49
TCACTGA22450.026.23336430-31
CCGTCTT22750.025.67958650-51
GTCACTG22600.025.63893728-29
CTCGTAT23150.025.44379642-43
GACCAAT22600.025.42878234-35
CTTGAAA23000.025.40228860-61
Produced by FastQC (version 0.11.2)