FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g1_ov.351000000b862f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g1_ov.351000000b862f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556364
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC107280.6892989043694149TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG18350.030.92928732-33
TATGCCG19150.029.89001746-47
GTATGCC19050.029.54726644-45
CTTAGGC19250.029.48324432-33
CACTTAG19400.028.7656530-31
CGTATGC20000.028.50002144-45
TAGGCAT20650.027.71437534-35
ATGCCGT20850.027.22511546-47
TCGTATG20950.026.98092542-43
TGCTTGA21450.026.4618758-59
TTAGGCA21900.026.24093834-35
TCACTTA21950.025.74841930-31
GCCGTCT22150.025.41116348-49
GCTTGAA22150.025.19672458-59
AGGCATC23350.024.40919136-37
CCGTCTT23450.024.305150-51
CTTGAAA23800.024.14840160-61
TGCCGTC24100.023.55216248-49
GGCATCT24150.023.2072636-37
TCTCGTA26000.022.37910340-41
Produced by FastQC (version 0.11.2)