FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g1_fb.351000000b8605.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g1_fb.351000000b8605.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460331
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC114820.7862601013057998TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT21750.027.73746346-47
GCCGTCT21750.027.62636848-49
CGTATGC21850.027.50181444-45
CCGTCTT23100.025.80444350-51
TATGCCG24150.025.66939546-47
TCGTATG23300.025.5864542-43
ATGTATC23950.025.4844536-37
GTATGCC24750.024.95114144-45
GCTTGAA24400.024.91711858-59
GGGGTAT1351.2946684E-524.7465111
TGCTTGA24550.024.57139858-59
CTCGTAT24900.024.22850642-43
GTATCTC25700.023.47310638-39
TCTGCTT25800.023.47297556-57
CTGCTTG25700.023.471956-57
TCTCGTA26400.023.12099840-41
CTTGAAA26700.022.9478160-61
AGTCACC26700.022.76913828-29
ACCGATG28000.022.47531332-33
TGTATCT26700.022.32595636-37
Produced by FastQC (version 0.11.2)