FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g1_br.351000000b85ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g1_br.351000000b85ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983080
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC207370.6951540019040723TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC39700.026.56132544-45
TATGCCG40450.026.18846346-47
TGCTTGA42150.025.12927858-59
TCGTATG44450.024.04351842-43
GCTTGAA43900.023.96524658-59
ATGCCGT44400.023.85863146-47
CGTATGC44650.023.77624944-45
GCCGTCT44950.023.51248448-49
CCGTCTT46700.023.39287850-51
CTGCTTG48600.021.84344956-57
CCAGTCA50000.021.18184926-27
CTCGTAT50750.021.152442-43
TGCCGTC50550.021.00170348-49
AGTCACA50700.020.98307228-29
CTTGAAA52550.020.60812460-61
TCTGCTT52100.020.60395856-57
GTCACAT52050.020.2107328-29
GTCTTCT53800.020.12947852-53
ACATCAC52900.020.02165632-33
CAGTCAC52250.019.99702526-27
Produced by FastQC (version 0.11.2)