FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d0_2_ov.351000000b87f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d0_2_ov.351000000b87f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6520958
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT23300.012.0266838
TCTAGAC24500.012.0191947
CGGGGTT9800.011.6906441
CGGGGAT20450.011.4381561
CTGGAAT37250.011.02111151
CTGGGAT21300.010.9817041
CCGGGGT9407.2759576E-1210.6646031
CGGGAAT31900.010.6248041
ATTCTAG33100.010.4745325
GGGAATC25750.09.9597012
CTCCGAT32450.09.8562871
CTCGGAT34150.09.7849941
CTCGAAT50000.09.5473581
CATTCTA40450.09.51034454
CCGGGAT16650.09.4613471
CGGGGAC18250.09.4165731
TTCTAGA36150.09.1966476
GGTCATA18600.09.1921974
CGGGGAG22200.09.0312851
CGCGGAT37350.08.9466541
Produced by FastQC (version 0.11.2)