FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d0_2_fb.351000000b87db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d0_2_fb.351000000b87db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4662009
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAT15900.021.916181
TCTAGAC25450.019.5946067
CTAGACT25350.019.1098488
ATCTAGG10650.018.7298685
CGGGGCT6000.015.9117461
TAGACTT31600.015.7810989
CATTCTA35550.015.6306344
TCTAGGA13200.015.4713976
TTCTAGA35900.014.68464856
ATTCTAG36550.014.1636155
CGGGGCA3501.0425907E-412.27477551
CTCGGAT27200.011.933811
GGTCATA14750.011.9135194
CGAGGAT40500.011.550751
CGGGACT12000.011.53601551
TGATCTA16500.011.5134743
CCGGGAT18000.011.1382241
GAACTAA24050.011.061172592-93
TCATTCT56700.010.9728293
CGGGGTT11900.010.8306861
Produced by FastQC (version 0.11.2)