FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d0_2_br.351000000b87b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d0_2_br.351000000b87b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751977
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCAT3305.620677E-1018.8028241
TCTAGAC11050.013.7540227
CTAGACT11450.013.6883318
CCGGGTT6259.458745E-1112.9826251
CCGGGAT9200.012.4513671
CTCAGAT10500.011.8189181
CATTCTA15700.011.7977484
ATTCTAG14200.011.3718885
CTGGCAT7602.0372681E-1011.3045311
CGGGCTT8459.094947E-1211.2970981
GTATGCC11150.011.28892244-45
CGGGCAC8054.7293724E-1111.2655221
CTTTTCT49700.011.2362951
GGTCATA9103.6379788E-1210.9600384
CGGGAAT14850.010.9281371
TAGACTT14350.010.9220489
CACCCGT12000.010.68642530-31
CTGGTAT9851.8189894E-1210.66056251
TTCTAGA16150.010.5869776
TCTCTTA21550.010.578797
Produced by FastQC (version 0.11.2)