FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w5_ov.351000000b87a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w5_ov.351000000b87a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241171
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT34850.10752286750683626TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT23400.014.004348
TCTCTTA25900.013.0201157
TATGCCG15250.012.46044348-49
GTATGCC15300.011.95472146-47
CGGGGTT6452.1555024E-911.83836651
CTCGGTT14450.011.2290391
CTTTTCT61800.011.1200191
CTGGTAT14750.010.6771031
CCCAGAT8055.6024874E-1010.6710741
ATGCCGT19200.010.63923848-49
CTGGGAT10850.010.5563331
CTAGACT11800.010.5153291
CCGGGAT9801.4551915E-1110.2264461
CCAGTCA18650.010.05883526-27
CTCGGGT8702.1518645E-99.873811
CGGGGAT11151.8189894E-129.8442921
CGGGAAT15250.09.7011531
CGTATGC18300.09.60551846-47
CTCAGAT14000.09.5446831
GCCGTCT20550.09.12987850-51
Produced by FastQC (version 0.11.2)