FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w5_fb.351000000b878b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w5_fb.351000000b878b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898163
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT19870.10468015655136044TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC12850.018.8509087
CTAGACT12700.018.6980888
ATTCTAG16750.016.4466865
TTCTAGA17200.015.187966
TAGACTT15950.015.1858949
CGGGGAC3806.488517E-815.0716761
GGTCATA6700.014.8870864
CATTCTA17250.014.5932474
AACCTAG2307.992753E-414.4555755
CTCATTT10900.014.4494511
GGGGACT2100.007297447413.570542
CCCGTAT3951.6459562E-613.2910571
CGCGGGG4052.1795004E-612.9628821
CCCGGGG3003.93142E-412.7271941
GAACTAA11050.012.46491992-93
TAATCCC8950.012.2049419
CTTTCGT20400.012.1657011
GGGAGAC5604.587673E-811.9317451
CTGGGTT2800.0033888911.9317451
CCGGGAT7651.8189894E-1111.853761
Produced by FastQC (version 0.11.2)