FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w5_br.351000000b8761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w5_br.351000000b8761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493059
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT28830.11564106585524049TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT19050.018.1993878
TCTCTTA23400.015.22277
GTATGCC11750.014.55686146-47
TCTAGAC9350.014.223057
TATGCCG13000.012.79104548-49
CGGGGAT9800.012.6598291
CTTTTCT46800.012.6431831
ATTCTAG13150.011.9188535
CCCAGAT6854.4019544E-1011.8423791
CCCGGGT4507.094748E-611.6643561
CCGGGAT9150.011.4731381
CGTATGC15950.011.31946846-47
CCAGTCA14950.011.27769626-27
CTAGACT10550.011.2542468
AGTCAAC15400.010.94936234-35
GCCGTCT16450.010.82763150-51
CTTAGTT5758.3178384E-710.7883761
GGGAATC9701.8189894E-1210.7720212
TCTTATT32350.010.7171039
CTGGGGT3150.00816321510.603961
Produced by FastQC (version 0.11.2)