FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w4_ov.351000000b8748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w4_ov.351000000b8748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642913
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT20100.014.1768168
CTAGACT10800.014.0718018
CGGGGAT7550.013.2735711
TCTCTTA23950.013.0883997
CCGGGGT3651.0678321E-513.0744241
CGGGAAT11800.012.9414631
TCTAGAC11750.012.53060157
CTCGGTT11850.012.4841431
CTGGGAT9200.011.9304121
CTAAGAT9150.011.4740561
GGGAATC9950.011.4560872
CTGGCTT10850.011.4356021
CTCAGAT11850.011.2761
ATTCTAG14400.010.8842765
CGGGGCG7201.2063538E-810.6048111
CTCCACT11900.010.42657851
CTGAGAT14300.010.3452521
GGTCATA7351.790795E-810.3390534
CTTTTCT49850.010.3388921
GGGACAT7904.654794E-910.2692161
Produced by FastQC (version 0.11.2)