FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w4_fb.351000000b872e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w4_fb.351000000b872e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648466
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA9150.017.648084
TCTAGAC15250.014.3260897
GTCATAA12400.014.1716275
CTAGACT16200.014.1355721
CTTTCGT26150.013.5004181
CAGGGGA3204.5272303E-513.4177511
TAATCCC14950.012.7074529
TAGAATA17000.012.5719682
CGGGGCG4951.1967131E-712.5292581
CTCGGTT9650.012.359471
CGTACAA27250.012.0260295
CTAGAAT23850.012.0019021
CGGGTCT6401.9153958E-911.9268891
TTTCGTA27500.011.9167012
CATTCTA20900.011.8167144
CTCCGAT13500.011.6618481
CTAGGAT5403.7067366E-711.4851541
CGGGTAT6653.4979166E-911.478511
TTCGTAC26900.011.476273
CAGGGAT5454.176418E-711.3797851
Produced by FastQC (version 0.11.2)