FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w4_br.351000000b86f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w4_br.351000000b86f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8077059
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT69750.022.60578
TCTCTTA82800.019.9035847
TCTAGAC35550.014.8291247
TCTTATT112600.014.2560649
CTAGACT36300.013.9991358
CTTTTCT164750.013.9879261
ATTCTAG45850.013.2587575
CTGGGAT26800.012.6402361
CTGGAAT40050.012.1514831
ATTTCCC70900.011.72234512-13
TTCTAGA50450.010.9201596
CTCGGAT43700.010.9181781
CATTCTA54400.010.6510664
CGGGGAT29650.010.4597251
CTTAGAT28150.010.33910751
CGGGAAT48500.010.1327451
CTGGGCT15600.010.0930161
CTCGAAT67450.09.9032491
TAGACTT53150.09.8290339
GTATGCC30850.09.62515244-45
Produced by FastQC (version 0.11.2)