FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w3_ov.351000000b86dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w3_ov.351000000b86dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803288
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC195891.0862934816845673TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC29500.031.08057244-45
TATGCCG30850.030.10462646-47
GCCGTCT31350.029.54749348-49
GCTTGAA31100.029.25081658-59
CGTATGC31550.029.21164944-45
TCGTATG32200.028.91784742-43
ATGCCGT32000.028.87428946-47
TGCTTGA31750.028.7267958-59
CCGTCTT32900.028.15114650-51
CTCGTAT34450.026.68440242-43
CTTGAAA34850.026.5122460-61
CTGCTTG36000.025.46703756-57
CAGATCT36000.025.46527136-37
TGCCGTC36650.025.27459748-49
TCTGCTT36750.024.94730256-57
TCTCGTA37850.024.28536840-41
GAGCACA75950.024.2632669
CCAGTCA38050.024.09061826-27
ATCAGAT38350.024.02734234-35
AGTCACG38300.023.99603828-29
Produced by FastQC (version 0.11.2)