FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w3_fb.351000000b86b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w3_fb.351000000b86b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043521
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC208891.0222062802388623TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT34500.030.56739846-47
CGTATGC35650.029.71460344-45
TATGCCG36000.029.4257146-47
TCGTATG36300.029.1839542-43
GTATGCC37100.028.80933444-45
CCGTCTT36500.028.75775150-51
GCCGTCT37300.028.4631348-49
TGCTTGA38200.027.5438358-59
CTGCTTG38650.027.40749256-57
CTTGAAA38450.027.3029760-61
GCTTGAA39800.026.6155758-59
TCTGCTT39800.026.4365456-57
TCACACT39800.026.31332630-31
CTCGTAT40000.026.30629242-43
TGAATCT40200.026.2887636-37
CACACTT40550.025.88520430-31
ACACTTG41050.025.39698832-33
GTCACAC42100.025.1014128-29
TCTCGTA42950.024.71885540-41
TGCCGTC43500.024.24251448-49
Produced by FastQC (version 0.11.2)