FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w3_br.351000000b8699.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w3_br.351000000b8699.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861091
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC231201.2422820807794996TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC35750.032.22333544-45
TATGCCG37300.030.81978646-47
ATGCCGT37750.030.57823446-47
TGCTTGA39200.029.3865658-59
GCTTGAA39900.028.93053458-59
GCCGTCT40100.028.84508748-49
TCGTATG40200.028.77526742-43
CGTATGC40450.028.71409444-45
CTCGTAT40450.028.36253542-43
CCGTCTT40650.028.33414550-51
CTGCTTG40850.028.25773456-57
GAGCACA81550.028.2461459
CAGATCA41700.027.79266534-35
AGAGCAC85350.026.9885548
AGATCAT42800.026.9673934-35
ACCAGAT43550.026.66549532-33
AGTCACC43900.026.34364728-29
TGCCGTC43750.026.33000648-49
CCAGTCA44200.026.27229526-27
TCTGCTT44350.026.18836256-57
Produced by FastQC (version 0.11.2)