FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w2_ov.351000000b868c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w2_ov.351000000b868c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2565462
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC118030.4600730784552646TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27500.023.06087546-47
GTATGCC28450.021.87339844-45
CGTATGC29500.021.65845544-45
GCCGTCT29850.020.92667248-49
ATGCCGT30300.020.85144646-47
CCGTCTT30700.020.65411650-51
GCTTGAA29950.020.61929158-59
CCAGTCA31050.020.34362626-27
TCGTATG31800.019.86866442-43
TGCTTGA31650.019.73691258-59
CAGTCAC32100.019.604226-27
AGTCACG32350.019.59970528-29
GCCAATA31650.019.50835434-35
TCACGCC32400.019.4232530-31
GAGCACA65150.019.3910489
CACGCCA32250.019.21904630-31
CTCGTAT33700.018.9599242-43
AGAGCAC67950.018.5221128
ACGCCAA34150.018.49763332-33
CCAATAT34450.018.26743934-35
Produced by FastQC (version 0.11.2)