FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w2_fb.351000000b8656.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w2_fb.351000000b8656.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4640244
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC190140.40976293488014853TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT42850.022.06232346-47
CGTATGC45000.020.79799344-45
GTATGCC46550.020.46267344-45
TATGCCG47450.020.02362846-47
ACAGTGA46700.019.73165934-35
TCGTATG47000.019.6604842-43
AGTGATC47600.019.60910636-37
CACAGTG48250.019.29426232-33
GTCACAC49400.019.03617128-29
CCGTCTT49750.018.99914750-51
GCCGTCT49650.018.89615448-49
CACACAG50650.018.2854630-31
TGCTTGA53150.018.14260358-59
ACACAGT52200.017.97074332-33
AGTCACA53100.017.84389728-29
CAGTGAT51950.017.82903734-35
GTGATCT53450.017.41849336-37
CAGTCAC54300.017.1432426-27
CTGCTTG57900.016.85968456-57
TCTGCTT58700.016.34667656-57
Produced by FastQC (version 0.11.2)