FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w2_br.351000000b8649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w2_br.351000000b8649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5381939
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC231070.4293434020712609TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC52450.023.868844-45
TATGCCG57650.021.92045446-47
ACTGACC58700.021.60436432-33
CGTATGC60200.021.2695244-45
CTGACCA60200.020.78990232-33
TGCTTGA62650.020.6625758-59
TCGTATG61050.020.50663642-43
CACTGAC61550.020.44823630-31
CCAATCT61800.020.44569236-37
CCAGTCA63250.020.1610826-27
CTCTTAT41850.020.0864758
ATGCCGT62900.020.05307846-47
TGACCAA64000.019.927534-35
CCGTCTT64750.019.80597950-51
GCTTGAA63600.019.75638658-59
ACCAATC64450.019.7155836-37
AGTCACT65200.019.59470428-29
GCCGTCT64300.019.5413148-49
GACCAAT64550.019.50015634-35
TCACTGA65950.019.30003230-31
Produced by FastQC (version 0.11.2)