FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w1_ov.351000000b8612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w1_ov.351000000b8612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415667
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC111830.46293632359095854TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG21850.025.9764632-33
CTTAGGC22200.024.81809632-33
CACTTAG22350.024.7565130-31
TAGGCAT22200.024.71240234-35
GTATGCC23800.023.55536844-45
CGTATGC24700.023.37029344-45
TATGCCG24400.023.26724446-47
TTAGGCA24650.022.93062834-35
AGGCATC25800.021.90988736-37
TCACTTA26300.021.39949830-31
ATGCCGT26100.021.29669646-47
CCGTCTT27900.020.25824250-51
TCGTATG27800.020.25176642-43
CCAGTCA28400.020.23522626-27
AGTCACT28650.020.05865128-29
GGCATCT27650.019.92855336-37
GCCGTCT29000.019.8210848-49
TGCTTGA29000.019.73917458-59
CTCGTAT29700.019.1961542-43
GCTTGAA29700.018.95403958-59
Produced by FastQC (version 0.11.2)