FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w1_fb.351000000b85f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w1_fb.351000000b85f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014522
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC130840.434032327513284TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC29400.022.4620144-45
ATGCCGT31200.021.69908346-47
TATGCCG31050.021.19187246-47
TCGTATG31500.021.11536642-43
GTATGCC31700.021.05708344-45
CCGTCTT31400.020.87567350-51
GCCGTCT32700.020.4119548-49
AGTCACC33900.019.33312428-29
ATCTAGG5900.019.3201585
CAGTCAC35150.018.64560126-27
TGCTTGA36250.018.21534758-59
ATGTATC36550.018.06523936-37
GTATCTC37600.017.81505638-39
TCTGCTT38750.017.53169356-57
CCAGTCA38100.017.45121626-27
GAGCACA77100.017.43239
CTGCTTG39850.017.10736356-57
CTAGGAT8400.017.0395181
GATGTAT40100.016.8204934-35
GTCACCG39700.016.74789228-29
Produced by FastQC (version 0.11.2)