FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d3_w1_br.351000000b85dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d3_w1_br.351000000b85dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210045
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC120990.5474549160763694TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC24000.027.91156244-45
TATGCCG25750.025.73790746-47
ATGCCGT27800.024.52355246-47
CGTATGC28450.023.3787944-45
GCCGTCT28900.023.34248548-49
TCGTATG29450.022.98876642-43
GCTTGAA29600.022.70867758-59
TGCTTGA29250.022.65559658-59
CCGTCTT30250.022.21795750-51
CTGCTTG31250.021.66191756-57
CCAGTCA31600.021.1909826-27
CTCGTAT33400.020.34115842-43
CAGTCAC33350.020.15021326-27
TCTGCTT33650.020.11693656-57
TGCCGTC33550.020.10723948-49
AGTCACA33500.019.98910328-29
GTCACAT34100.019.91593628-29
CACATCA34500.019.61619630-31
CATCACG35550.019.30618332-33
ACATCAC34850.019.28509732-33
Produced by FastQC (version 0.11.2)