FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d0_5_ov.351000000b8854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d0_5_ov.351000000b8854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5770181
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT45250.015.3236698
TCTCTTA52100.014.8593887
TCTAGAC23550.012.3024047
CTAGACT23100.012.1302168
CTCGGAT27100.010.5659331
CTTTTCT114500.010.0030331
CGGGGTT8601.7644197E-99.9884931
CTCGAAT44500.09.8662871
TTCTAGA35350.09.5393776
CGGCAAT34250.09.4748921
ATTCTAG31700.09.4391375
CGGGCAT15250.09.3880921
CCCAATT25950.09.3790471
CATTCTA37950.09.1361274
ATTTCCC41850.09.13546812-13
CGGGGAT17850.09.0900571
CGGGAAT29500.09.0592441
TCTTATT82850.08.7705619
CCGGGAT18100.08.7008431
CGCGGAT35900.08.6406191
Produced by FastQC (version 0.11.2)