FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d0_5_fb.351000000b8847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d0_5_fb.351000000b8847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947990
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTGT3051.6613267E-615.646671
CTAGACT20800.015.297738
CTAGGAT7200.015.2446371
TCTAGAC22550.014.95410257
GGTCATA16600.013.7335164
CATTCTA29450.012.9018884
GTCATAA18300.012.4577255
CTCGGAT24150.012.4493071
TAGACTT26800.012.4044879
ATTCTAG31050.012.3900185
TTCTAGA29750.012.1331956
GAGGTCA19450.011.9653392
GGGGCGT2800.003515816511.8737692
CGCGGGG8450.011.859991
TAATCCC19500.011.6902079
ATCTAGG6956.0572347E-1011.61756
CGGGGGG3701.7541568E-411.6081381
AACTAGC7903.6379788E-1111.4228666
CGAGGAT35000.010.6352651
CGAGGTC25000.010.3080251
Produced by FastQC (version 0.11.2)