FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d0_5_br.351000000b8810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d0_5_br.351000000b8810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5675222
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT128670.22672240839212987No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA91080.16048711398426352No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT81220.1431133442885582No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT74990.13213580015019677No Hit
CCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCCTAAGGT61890.10905300268430028No Hit
CTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAACAGA60690.10693854795459984No Hit
CATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCC57790.1018286156911571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT60750.029.4726728
GGTCATA35800.025.7381654
TCTCTTA76350.023.9502777
AGGTCAT37350.021.3637543
GAGGTCA38650.020.6451822
GTCATAA48800.019.3683285
ATTTCCC56600.016.94959612-13
TAATCCC56350.016.5191089
CGAGGTC48750.016.1509171
CTTTTCT159650.015.2436551
CGGGGTC11850.014.8994851
GGCCTTA20250.014.54307236-37
TTCGTAC95600.014.4575253
GGGGTCA9550.014.4229312
CGTACAA100050.013.9094325
TCTTATT130950.013.8179599
CTTTCGT117500.013.523661
ATAATCC69400.013.4812828
TCTAGAC38450.013.4644647
GGGGAAT20000.013.3612141
Produced by FastQC (version 0.11.2)