FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d0_1_ov.351000000b8803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d0_1_ov.351000000b8803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381797
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCT3151.2751116E-716.6613831
CGGGTCT5752.3646862E-1114.1061991
CTCTTAT18600.013.532328
CGGGGAT8150.013.4647761
TCTCTTA20400.013.2708777
CGGGCAT7053.6379788E-1212.8585911
CGGGGTT3801.6192042E-512.5558271
GGGGGAT4606.6884604E-712.4466461
CGGGGAC7301.0186341E-1011.7646371
CGGGCTT7501.6370905E-1011.4509131
CTAGACT8851.8189894E-1211.3215261
GGGGATC5052.1785181E-611.2861222
GGGCATC6151.7969433E-710.8120452
CTCAAAC8952.910383E-1110.6619321
CTGGAAT15800.010.5691451
CTCGGGT5455.142616E-610.5054261
CGGGATT10101.8189894E-1210.3927441
CTCGAAT15650.010.3655781
CTTTTCT44250.010.3511091
CCCGGAT4651.268183E-410.2606751