FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d0_1_fb.351000000b87e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d0_1_fb.351000000b87e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033038
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT43090.1420687772457846TruSeq Adapter, Index 18 (97% over 40bp)
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC30700.10121864612312803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAT10200.022.9248281
CTAGACT15950.017.3531171
TCTAGAC15900.017.3253217
ATCTAGG7200.015.1721015
GGTCATA9750.015.1010634
GGGGAAT7350.014.9331251
GTCCGCA15750.014.02380334-35
TAGACTT20850.013.439912
TCTAGGA8250.013.2411076
CATTCTA22950.012.8309684
ATTCTAG23800.012.7718395
CACATCT18200.012.52849938-39
ATGCCGT18400.012.26282948-49
TAGGATT22500.012.2432272
GCACATC18250.012.23387938-39
CCGGGGG2750.00296467712.1471811
GCCGTCT19550.011.90477650-51
TTCTAGA23900.011.7247756
CTCGGAT21600.011.7093371
CGCACAT18900.011.68688836-37
Produced by FastQC (version 0.11.2)