FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l01n01_d0_1_br.351000000b87ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l01n01_d0_1_br.351000000b87ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461481
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC32640.1326030954535095TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC10200.016.7642737
CTCTTAT20500.016.2171158
CTGGGTT4156.8394E-1016.0966051
CTAGACT10350.016.0604298
TCTCTTA22750.015.24136457
CACCCGT12950.014.85301830-31
GTATGCC13950.014.64357844-45
ATTCTAG12550.014.3820975
CTCAGAT9750.014.1921351
ACCCGTC14150.013.09046932-33
GTCACCC15000.012.82310528-29
TATGCCG16000.012.61839946-47
CCGGGTT5704.3764885E-912.5565631
CCAGTCA15400.012.3358426-27
CATTCTA16200.012.3144964
CCCGTCC15950.012.20872432-33
TCACCCG15600.012.17768930-31
TTCTAGA14900.012.113786
AGTCACC16300.012.09177128-29
CGGGAAT13600.011.9287351
Produced by FastQC (version 0.11.2)