FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7460233
Sequences flagged as poor quality0
Sequence length74
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4860296.514930565841576No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGACCATCATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTA397100.5322890049144577Illumina RNA PCR Primer (96% over 31bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285970.38332582909943963No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACCATCATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAA245180.32864925264398576Illumina RNA PCR Primer (96% over 31bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGATTGCTGCCGAGTGTAGATCTCGGTGGTCGCCGTATCATTA237200.3179525357988149Illumina Single End PCR Primer 1 (100% over 31bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGTAGAGCCGGTGTAGATCTCGGTGGTCGCCGTATCATTA218520.2929131033843045Illumina Single End PCR Primer 1 (100% over 29bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGATCGTGCATTCGTGTAGATCTCGGTGGTCGCCGTATCATTA150630.20191058375790674Illumina RNA PCR Primer (100% over 30bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGACTATAGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTA142760.19136131539055148Illumina RNA PCR Primer (96% over 30bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGATTGCTGCCGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA140810.1887474560110924Illumina Single End PCR Primer 1 (100% over 31bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGATCGTGCATTCGTGTAGATCTCGGTGGTCGCCGTATCATTAA121110.16234077407501882Illumina RNA PCR Primer (100% over 30bp)
ACTCTCTCTTATACACATCTGACGCTGCCGACGACCATCATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTA96370.12917827097357415Illumina RNA PCR Primer (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)