FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n02_832.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n02_832.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37219126
Sequences flagged as poor quality0
Sequence length74
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGACCATCATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTA28438557.640843043976905Illumina RNA PCR Primer (96% over 31bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGACCATCATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAA12995903.491726269982804Illumina RNA PCR Primer (96% over 31bp)
TCTGTCTCTTATACACATCTGACGCTGCCGACGACCATCATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTA2348120.630890687760911Illumina RNA PCR Primer (96% over 31bp)
CCTGTCTCTTATACACATCTGACGCTGCCGACGACCATCATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTA666550.17908803124501096Illumina RNA PCR Primer (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)