FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n02_831.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n02_831.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33560289
Sequences flagged as poor quality0
Sequence length74
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGATTGCTGCCGAGTGTAGATCTCGGTGGTCGCCGTATCATTA13907594.14406145310608Illumina Single End PCR Primer 1 (100% over 31bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGATTGCTGCCGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA7023412.0927739925004816Illumina Single End PCR Primer 1 (100% over 31bp)
TCTGTCTCTTATACACATCTGACGCTGCCGACGATTGCTGCCGAGTGTAGATCTCGGTGGTCGCCGTATCATTA1760120.5244650902738055Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)