FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n02_830.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n02_830.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22279655
Sequences flagged as poor quality0
Sequence length74
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGACTATAGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTA12051435.409163651771089Illumina RNA PCR Primer (96% over 30bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGACTATAGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAA4274751.9186787228078712Illumina RNA PCR Primer (96% over 30bp)
TCTGTCTCTTATACACATCTGACGCTGCCGACGACTATAGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTA949830.4263216822702147Illumina RNA PCR Primer (96% over 30bp)
CCTGTCTCTTATACACATCTGACGCTGCCGACGACTATAGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTA369490.16584188579221715Illumina RNA PCR Primer (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)