FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7460233
Sequences flagged as poor quality0
Sequence length74
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGC458290.6143105718011757No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGAAT254760.34149067462102056No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGG200680.26899964116402264No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCATT161660.21669564476069314No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAGCG161240.2161326596635789No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTTA153280.20546275163255626No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGCAACCATCTCGTATGCCGTCTTCTGCTTGAAAAA151510.20309017158043188RNA PCR Primer, Index 36 (96% over 29bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAGCGGCACATCTCGTATGCCGTCTTCTGCTTGAAAAA108160.14498206691399587Illumina PCR Primer Index 9 (96% over 27bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAGC99410.13325321072411545No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCATTGCCGATCTCGTATGCCGTCTTCTGCTTGAAAAA86930.11652451069557745RNA PCR Primer, Index 33 (96% over 28bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA81850.10971507190190977Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)