FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n01_832.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n01_832.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37219126
Sequences flagged as poor quality0
Sequence length74
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGCAACCATCTCGTATGCCGTCTTCTGCTTGAAAAA17409194.677484903863674RNA PCR Primer, Index 36 (96% over 29bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGCAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA5393551.4491339748278882RNA PCR Primer, Index 36 (96% over 29bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGCAACCATCTCGTATGCCGTCTTCTGCGTGAAAAA1726100.4637669353116997RNA PCR Primer, Index 16 (96% over 26bp)
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGCAACCATCTCGTATGCCGTCTTCTGCTTGAAAAA1515690.40723417309691795RNA PCR Primer, Index 36 (96% over 29bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGCAACCATCTCGTATGCCGTCTTCTGCGTGAAAAAA864990.23240470504331565RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGCAACCATCTCGTATGCCGTCTTCGGCTTGAAAAA390570.104937982692017RNA PCR Primer, Index 16 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)