FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n01_831.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n01_831.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33560289
Sequences flagged as poor quality0
Sequence length74
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA7836832.335149736046671Illumina PCR Primer Index 10 (96% over 28bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAA2586860.7708098103684388Illumina PCR Primer Index 10 (96% over 28bp)
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA976870.29107913820408404Illumina PCR Primer Index 10 (96% over 28bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCGTGAAAAA819540.24419932736574468RNA PCR Primer, Index 35 (96% over 26bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCGTGAAAAAA454990.13557392190514211RNA PCR Primer, Index 35 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)