FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n01_829.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n01_829.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29141166
Sequences flagged as poor quality0
Sequence length74
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCATTGCCGATCTCGTATGCCGTCTTCTGCTTGAAAAA9420583.232739554759065RNA PCR Primer, Index 33 (96% over 28bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCATTGCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA4961191.7024679108584744RNA PCR Primer, Index 33 (96% over 28bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCATTGCCGATCTCGTATGCCGTCTTCTGCGTGAAAAA449840.1543658205028584Illumina Paired End PCR Primer 2 (96% over 27bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)