FastQCFastQC Report
Thu 28 Oct 2021
H57VYAFX3_n01_827.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VYAFX3_n01_827.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27379747
Sequences flagged as poor quality0
Sequence length74
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAGCGGCACATCTCGTATGCCGTCTTCTGCTTGAAAAA10141373.7039677539752285Illumina PCR Primer Index 9 (96% over 27bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAGCGGCACATCTCGTATGCCGTCTTCTGCGTGAAAAA931250.34012366878335293Illumina PCR Primer Index 11 (96% over 26bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAGCGGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAA803090.29331534728936687Illumina PCR Primer Index 9 (96% over 27bp)
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAGCGGCACATCTCGTATGCCGTCTTCTGCTTGAAAAA339790.12410268071505555Illumina PCR Primer Index 9 (96% over 27bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)