FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_hy_ant7_mut.351000000b8b1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_hy_ant7_mut.351000000b8b1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7140542
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1149201.609401639259317TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC140500.040.1929846-47
GAGCACA284250.039.847889
TATGCCG145850.038.78363448-49
ATGCCGT147000.038.39945248-49
AGAGCAC300300.037.8446668
CGTATGC151550.037.4190846-47
TCGTATG151950.037.22667344-45
TGCTTGA151650.036.97315260-61
GGAATCT155050.036.17542638-39
CTGCTTG159900.035.30316558-59
GCTTGAA160800.035.10557660-61
CCGTCTT163200.034.61587552-53
AAGAGCA335950.033.8852547
TTCGGAA167350.033.8567936-37
CTTGAAA166900.033.7798262-63
TCTGCTT167000.033.74537358-59
TCGGAAT168000.033.485536-37
CCAGTCA169450.033.46581326-27
CGGAATC170050.033.2497538-39
GTCACGT170600.033.11470828-29
Produced by FastQC (version 0.11.2)