FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_hy_ant7_con.351000000b8af2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_hy_ant7_con.351000000b8af2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8888200
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1638081.8429828311694156TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA383550.042.8802879
AGAGCAC404600.040.743278
GTATGCC202250.040.54303446-47
GCCTTAT206900.039.7809436-37
TATGCCG206750.039.74077648-49
GTGGCCT212900.038.58109334-35
ATGCCGT213150.038.5586748-49
GGCCTTA214150.038.33436636-37
CGTATGC218300.037.56220246-47
CCTTATC218600.037.50010738-39
TGCTTGA219350.037.341360-61
AAGAGCA445700.037.018127
TCGTATG221250.036.95414744-45
TGGCCTT224500.036.78856734-35
CCGTCTT226700.036.26395852-53
CTTATCT226550.036.1527238-39
GCTTGAA228150.036.00509660-61
CTGCTTG227900.035.9924958-59
CCAGTCA232600.035.15151626-27
CTCGTAT233100.035.11627644-45
Produced by FastQC (version 0.11.2)