FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_hy_ant3_wt.351000000b8b37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_hy_ant3_wt.351000000b8b37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5625078
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT878401.5615783461136006TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA183850.047.7376489
AGAGCAC192400.045.789048
AAGAGCA216150.040.779837
TATGCCG113500.038.72743248-49
GTATGCC113150.038.6796746-47
ACCGTAC114150.038.42420632-33
AGTCACC117500.037.45146628-29
ATGCCGT118100.037.15867648-49
CCAGTCA118250.037.13360226-27
GAAGAGC237150.037.1238146
CAGTCAC119400.036.69639226-27
CGTATGC121600.036.24569346-47
TCGGAAG243150.036.1336173
GCCGTCT121250.036.0754850-51
TCGTATG120950.036.04780244-45
TGCTTGA122350.035.9073760-61
GTAATCT121350.035.87027438-39
CCGTACG122050.035.7425332-33
CGGAAGA247700.035.5467384
TCCAGTC123600.035.50422724-25
Produced by FastQC (version 0.11.2)