FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g5_ov.351000000b8a7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g5_ov.351000000b8a7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521245
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT54820.21743226064900475TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC13650.020.8770546-47
TATGCCG14700.019.54738248-49
ATGCCGT16650.017.25804948-49
CGTATGC17250.017.20843946-47
CCAGTCA17400.016.3786626-27
ATGTCAG18250.015.87482434-35
CATGTCA19100.015.16895332-33
GTCACAT19300.015.13540928-29
GTCAGAA20000.015.07930636-37
GAGCACA38000.014.9979199
AGAGCAC37600.014.9051418
CCGTCTT19450.014.895452-53
CAGTCAC18950.014.78833226-27
AGTCACA19450.014.77447928-29
CTCTTAT15700.014.5205658
GCCGTCT19650.014.50223550-51
TCACATG20000.014.48692530-31
TCGTATG19850.014.35625844-45
TGCTTGA21500.013.917500560-61
CTCCAGT20750.013.73303324-25
Produced by FastQC (version 0.11.2)