FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g5_fb.351000000b8a52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g5_fb.351000000b8a52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940660
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT46520.1581957791788238TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA8300.016.6308734
ATCTAGG6400.015.6153975
GTATGCC15350.015.47071746-47
CTAGACT15650.015.173388
CTAGGAT8850.015.0653821
CTGGGTT5258.54925E-1114.5119591
CGTATGC17650.014.39653246-47
TATGCCG17650.014.3964148-49
TCTAGAC16750.014.1769197
TCACAGT19150.013.39241830-31
ATGCCGT17600.013.35788348-49
CCGTATC18000.013.32456138-39
GTCACAG18500.012.83629628-29
CGGGCCT5001.398439E-712.3805141
TCCGTAT19400.012.36278636-37
CCGGGGG2700.002626177612.3452431
TCTAGGA7801.8189894E-1212.1827896
TAGACTT19550.012.1464669
GCCGTCT20650.012.07482150-51
AGTCACA21350.012.012609528-29
Produced by FastQC (version 0.11.2)